We sought to evaluate in vivo and in vitro left ventricular lv geometry and function in streptozotocininduced diabetic rats and the possible role of the nitric oxide no pathway. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. Asparagus officinalis extract controls blood glucose by improving insulin secretion and. In our experiments, we followed the rats with streptozotocin induced diabetes for one year and a recovery was observed in a significant portion of animals. Diabetes was induced by streptozotocin 40 mgkg in female wistar rats. Streptozotocininduced diabetic models in mice and rats article in current protocols in pharmacology chapter 5supplement 40. Quercetin liposomes ameliorate streptozotocininduced diabetic. The timecourse changes in the blood glucose levels during the oral glucose tolerance test 0120 min of all groups were shown in figure 2. Novel mechanism for plasma glucoselowering action of. Wound healing in streptozotocininduced diabetic rats. Alloxaninduced diabetes, a common model for evaluating the. Hypoglycemic effects of gynura procumbens fractions on streptozotocininduced diabetic rats involved phosphorylation of gsk3. Evaluation of antidiabetic and hypolipidemic effect of.
Sep 01, 2015 streptozotocin stz is an antibiotic that produces pancreatic islet. Changes in oxidative stress and antioxidant enzyme activities. Progression of early phase diabetic nephropathy in. Streptozotocin stz, 60 mgkg was injected intraperitoneally in rats to induce diabetes. Evaluation of antidiabetic and hypolipidemic effect of cucumis sativus fruit in streptozotocininduced diabetic rats. Quercetin liposomes ameliorate streptozotocininduced. Streptozotocininduced diabetic models in mice and rats. Nondiabetic n and streptozotocininduced diabetic d rats underwent 4h infusion of saline or sstr2a with insulininduced hypoglycemia clamped at 2. Detailed in this unit are protocols for producing stz. Murugesan 2 1 department of biochemistry, kanchi shri krishna college of arts and science, kilambi. Antihyperglycemic effect of catalpol in streptozotocin. Bitter melon is an excellent source of vitamins and minerals that.
Altered kidney morphology and enzymes in streptozotocin induced. Wound healing in streptozotocininduced diabetic rats using. Antioxidant profile in diabetic rats treated with a. Murugesan2 1department of biochemistry, kanchi shri krishna college of arts and science, kilambi, krishnapuram, kancheepuram, tamil nadu, india. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells. We studied the effects of streptozotocin stzinduced diabetes on kidney morphology, anatomy. This study aimed at investigating the acute phase of pathological changes in streptozotocin induced diabetic rats with varying glucose levels. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for. Aqueous anise extract alleviated the pancreatic changes in streptozotocininduced diabetic rat model via modulation of hyperglycemia, oxidative stress, apoptosis and autophagy.
After 5 days, dp extract 25 mgkgday and metformin 50 mgkgday were administered orally to diabetic rats for 28 days. In the in vitro insulin secretion studies on isolated. Infertility is a common complication in diabetic men and experimental animals, mainly due to loss of germ cells by apoptotic cell death. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. Momordica charantia linn cucurbitaceae mc is used in folk medicine to treat various diseases including diabetes mellitus. The present study was aimed to evaluate the anti diabetic potential of terminalia chebula t. The compound has the molecular formulae, c 4 h 2 n 2 o 4 and a relative molecular mass of 142.
Detailed in this unit are protocols for producing stzinduced insulin deficiency and hyperglycemia in mice and rats. Anti diabetic and hypolipidaemic properties of ginger zingiber officinale in streptozotocin induced diabetic rats volume 96 issue 4 zainab m. Hypoglycemic effects of gynura procumbens fractions on. Streptozotocininduced diabetic neuropathy in mice and rats. Poaceae leaves in streptozotocin induced diabetic rats. Alloxan which is chemically known as 5,5dihydroxyl pyrimidine2,4,6trione is an organic compound, a urea derivative, a carcinogen and cytotoxic glucose analog. Abnormal cardiac function in the streptozotocininduced.
The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Pdf brain indoleamines in alloxan and streptozotocin. Effect of aqueous extract and oil of moringa oleifera seed on blood glucose, serum alt and ast activities in streptozotocin induced diabetic rats prolonged treatment with aqueous extract and oil of moringa oleifera seed has shown a significant decrease p0. The results showed a significant decrease in liver protein concentrations in diabetic control rats, compared to those in the normal control rats and rats treated with. Animals will be injected with streptozotocin stz ip or iv once after fasting for 1218hrs animals will have free access to food after stz administration. Antidiabetic effects of aqueous extract and oil of moringa. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of. Male wister rats were randomly assigned to 4 groups. Pdf antidiabetic activity of fruits of terminalia chebula. Insulin secretagogue, alphaglucosidase and antioxidant. To better understand the insulinindependent plasma glucoselowering action of metformin, we used streptozotocin stzinduced diabetic rats to investigate the possible mechanisms. Male sprague dawley rats were used to establish a stz induced dn model. The streptozotocin diabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis. In rsvtreated diabetic rats, the aforementioned abnormalities were all attenuated. Myristica fragrans, parmelia perlata, illicium verum, trachyspermum copticum and myristica malabarica, the commonly used spices in india were assessed for antidiabetic activity in streptozotocin induced diabetic rats. Stz was given in a dose of 30 mgkg for 2 consecutive days intraperitoneally ip.
Streptozotocininduced diabetic ratsmice stz model advantages. A significant increase in urine sugar, urine volume, fasting blood glucose, total cholesterol, triglycerides and low density lipoprotein, and decrease in high density lipoprotein were observed in the rats. Thus, a selective somatostatin receptor type 2 antagonist sstr2a should improve hormone counterregulation to hypoglycemia. Bolus intravenous injection of 1 showed antihyperglycemic activity in a dosedependent manner in stz diabetic rats. The present investigation was undertaken to study the effects of chronic oral ramipril 1 mgkg treatment in streptozotocin stz induced diabetic rats. The effects of quercetin liposomes qpegl on streptozotocin stz induced diabetic nephropathy dn was investigated in rats. The longterm effects of streptozotocin and possible recovery from streptozotocin induced diabetes are explored much less.
Neurodegeneration in streptozotocininduced diabetic rats is. The levels of fasting blood glucose fbg, blood urea nitrogen bun. Streptozotocin induced diabetic neuropathy in mice and rats induction. Evaluation of antidiabetic properties of momordica charantia in streptozotocin induced diabetic rats using metabolomics approach abstract momordica charantia, also known as bitter melon or peria katak in malaysia, is a member of the family cucurbitaceae. Effect of tinospora cordifolia guduchi root extract on cardiotoxicity in streptozotocin induced diabetic rats. Antihyperglycemic and antioxidant activity of fructus hordei germinatus extract on streptozotocininduced diabetic rats. Stz induced diabetic male rats longdom publishing sl. Research article in streptozotocin induced diabetic rats. Siddiqui, paramdeep bagga1, supriya roy 1faculty of pharmacy, integral university, dasauli, kursi road, lucknow226026. Diabetic nephropathy dn is one of the most important complications of diabetes, and the leading cause of endstage renal disease esrd. The antihyperglycemic effect of catalpol 1 purified from the roots of rehmannia glutinosa was investigated in streptozotocin induced diabetic rats stz diabetic rats representing insulindependent diabetes mellitus.
Evaluation of antidiabetic properties of momordica charantia. Progression of early phase diabetic nephropathy in streptozotocininduced diabetic rats. Effects of stzinduced diabetes on the relative weights of kidney, liver and pancreas in albino rats. While chromium picolinate crpic supplementation has been found to be effective in treating diabetes, its effects on diabetic induced nephropathy have not been studied. In this study, we used an argonbased round atmosphericpressure plasma jet appj for enhancing wound healing in streptozotocin stz induced diabetic rats.
In stzinduced diabetic rats, it was shown that treatment with rsv significantly reduced malondialdehyde. Streptozotocin use in mice and rats version 1 date of publication. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Therapeutic effects of balanitoside in streptozotocininduced. Aug 15, 2018 in this study, we used an argonbased round atmosphericpressure plasma jet appj for enhancing wound healing in streptozotocin stz induced diabetic rats. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Evaluation of antidiabetic activity of bambusa vulgaris. In conclusion the use of quercetin liposomes allows to reduce disease symptoms in a rat model of dn. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. Ser9 in liver kesan hipoglisemik fraksi gynura procumbens di dalam tikus diabetes aruhan streptozotocin melibatkan pemfosfatan gsk3.
Brain indoleamines in alloxan and streptozotocininduced diabetic rats. The blood glucose levels reached the maximum after 30 minutes of administration of 2 g glucosekg bw and then reduced to initial levels within 2 hours in all groups except diabetic rats that showed severe glucose intolerance throughout the experimental. Pdf the objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of. The preliminary phytochemical study showed the presence of phytosterols and tannins. Effect of bauhinia holophylla treatment in streptozotocininduced. Research article open access dracaena arborea alleviates. Asparagus officinalis extract controls blood glucose by.
Dp administration protected both body and organ weight loss in stztreated diabetic rats. Protective effects of chromium picolinate against diabetic. Altered kidney morphology and enzymes in streptozotocin. This study was carried on 30 male spraguedawley rats weighing 8090 g, 60 days old, classified into 3 groups, control, diabetic and treated diabetic group. This study aimed to investigate the protective effects of allicin on renal damage in streptozotocin stz.
Eighteen rats, six control, six d1 group with glucose level of 200300 mgdl, and six d2 group with glucose level over 300 mgdl, were used. This study investigates the antidiabetic activities of momordica charantia bitter gourd on streptozotocin induced type 2 diabetes mellitus in rats. Pdf induction of diabetes by streptozotocin in rats researchgate. Antidiabetic and hypolipidaemic properties of ginger. Induction of diabetes by streptozotocin in rats ncbi nih. Streptozotocin stz was used to establish diabetic rat model. Effect of tinospora cordifolia guduchi root extract on. Icariside ii ameliorates diabetic nephropathy in streptozotocin induced diabetic rats wenjie tian,1,2, hongen lei,1, ruili guan,1 yongde xu,1 huixi li,1 lin wang,1 bicheng yang,1 zhezhu gao,1 zhongcheng xin1 1andrology center, peking university first hospital, peking university, beijing, 2department of urology, the second hospital of jilin university, jilin university, changchun, people.
Oral intake of metformin decreased the plasma glucose of stzinduced diabetic rats with a parallel increase of plasma. Evaluation of antidiabetic and hypolipidemic effect of cucumis sativus fruit in streptozotocininduceddiabetic rats. Ser9 hepar chong ching june, lee hui wen, halimah abdullah sani, jalifah latip. Effects of stzinduced diabetes on the relative weights of. Diabetes was confirmed by monitoring the blood glucose level. The present study was carried out to evaluate the anti diabetic activity of petroleum ether extract of bambusa vulgaris family. Altered kidney morphology and enzymes in streptozotocin induced diabetic rats morfologia y enzimas renales alteradas en ratas con diabetes inducida por estreptozotocin. Mar 25, 2011 spices are extensively used to enhance the taste and flavor of foods and are known to possess several medicinal properties. Streptozotocininduced diabetic neuropathy in mice and rats induction.
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